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vimentin apex2 in pecfp  (Addgene inc)


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    Addgene inc vimentin apex2 in pecfp
    Vimentin Apex2 In Pecfp, supplied by Addgene inc, used in various techniques. Bioz Stars score: 91/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/vimentin+apex2+in+pecfp/pm41894480-199-7-17?v=Addgene+inc
    Average 91 stars, based on 4 article reviews
    vimentin apex2 in pecfp - by Bioz Stars, 2026-08
    91/100 stars

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    Genomic organization and expression of F. novicida strains with ALFA tagged FPM13 and FLAG tagged APEX2 with or without a signal sequence (SS) for secretion. (A) Gene cassette of FLAG-tagged APEX2 with or without the first 24 amino acids of the F. novicida beta-lactamase (BlaB) driven by the promoter of F. tularensis LVS bacterioferritin (bfr) was inserted into the blaB locus. The cleavage site of the BlaB signal peptide sequence is indicated with a downward arrow. (B) Proteins from strains grown in TSBC (lanes 2, 3) or TSBC containing 5% KCl (lanes 4, 5) were separated on SDS-PAGE and the protein profiles imaged by stain free UV imaging (left panel). The proteins were transblotted onto nitrocellulose membrane and probed with FLAG-HRP to detect the expression of APEX2 and ssAPEX2 (middle panel) or with ALFA-HRP to detect the expression of FPM13 (right panel). Lane 1, molecular mass standards.

    Journal: bioRxiv

    Article Title: Discovery and CryoEM Structure of FPM13, a Periplasmic Metalloprotein Unique to Francisella

    doi: 10.1101/2025.05.08.652791

    Figure Lengend Snippet: Genomic organization and expression of F. novicida strains with ALFA tagged FPM13 and FLAG tagged APEX2 with or without a signal sequence (SS) for secretion. (A) Gene cassette of FLAG-tagged APEX2 with or without the first 24 amino acids of the F. novicida beta-lactamase (BlaB) driven by the promoter of F. tularensis LVS bacterioferritin (bfr) was inserted into the blaB locus. The cleavage site of the BlaB signal peptide sequence is indicated with a downward arrow. (B) Proteins from strains grown in TSBC (lanes 2, 3) or TSBC containing 5% KCl (lanes 4, 5) were separated on SDS-PAGE and the protein profiles imaged by stain free UV imaging (left panel). The proteins were transblotted onto nitrocellulose membrane and probed with FLAG-HRP to detect the expression of APEX2 and ssAPEX2 (middle panel) or with ALFA-HRP to detect the expression of FPM13 (right panel). Lane 1, molecular mass standards.

    Article Snippet: APEX2 sequence was amplified by PCR from Vimentin - APEX2 in pECFP, a gift from Alice Ting (Addgene plasmid #66170).

    Techniques: Expressing, Sequencing, SDS Page, Staining, Imaging, Membrane

    (A) FPM13 is biotinylated in the presence of H 2 O 2 and pulled down by streptavidin-agarose APEX2 that is targeted to the periplasm (ssAPEX2), but not by cytosolic APEX2. GroEL and KatG serve as cytosolic and periplasmic control proteins, respectively. (B - C) TX-114 phase extraction shows that FPM13 and KatG are soluble proteins that partition into the aqueous phase, unlike the known lipoprotein, IglE (C, lane 4). FPM13-ALFA is not secreted into the culture filtrate (C, lane5), unlike positive control IglC ( D , lane 6). Stain free UV-image shows equal loading of lanes being compared (E).

    Journal: bioRxiv

    Article Title: Discovery and CryoEM Structure of FPM13, a Periplasmic Metalloprotein Unique to Francisella

    doi: 10.1101/2025.05.08.652791

    Figure Lengend Snippet: (A) FPM13 is biotinylated in the presence of H 2 O 2 and pulled down by streptavidin-agarose APEX2 that is targeted to the periplasm (ssAPEX2), but not by cytosolic APEX2. GroEL and KatG serve as cytosolic and periplasmic control proteins, respectively. (B - C) TX-114 phase extraction shows that FPM13 and KatG are soluble proteins that partition into the aqueous phase, unlike the known lipoprotein, IglE (C, lane 4). FPM13-ALFA is not secreted into the culture filtrate (C, lane5), unlike positive control IglC ( D , lane 6). Stain free UV-image shows equal loading of lanes being compared (E).

    Article Snippet: APEX2 sequence was amplified by PCR from Vimentin - APEX2 in pECFP, a gift from Alice Ting (Addgene plasmid #66170).

    Techniques: Control, Extraction, Positive Control, Staining